How are Wolbachia – Aedes aegypti mosquito made in female form? Deng Lu, an NEA scholar, demonstrates the process to CNA. & nbsp,
First action: producing eggs
The child insectary room produces mosquito eggs, which are then used to produce adult Wolbachia mosquitoes in the future. & nbsp,
There are several malaria bars in the room, each housing a male and female mosquitoes. Many chicken strips and standing waters are kept in ovipots, or ovum collection containers, at the bottom of the cage.
According to Mr. Deng Lu, older scientist and head of the malaria production branch, mosquitoes enjoy the strips’ rough texture and will get on them to lay eggs. & nbsp,
Hatching hens and counting eggs are steps two.
A new batch of Wolbachia-carrying Aedes mosquitoes eggs hatch and develop into eggs in a matter of hours. The next step is to count them.
According to Mr. Deng, there are no bespoke solutions for mosquitoes production, particularly for counting larvae. The team first counted the larvae one by one using telecounters and droppers. & nbsp,
It is clear that the process was never long-lasting. & nbsp,
In order to qualify 26, 000 eggs in three to five minutes, the group created its own counting device.
Step 3: Egg should be seeded.
The seeding procedure is the following action. The 26, 000 larvae in each batch are channeled into specific trays after being poured through seeding pods. A high-density nurturing rack system is then used to load the boxes.
With controlled humidity, temperature, and air, the area has been created to be comfortable for malaria eggs. & nbsp,
According to Mr. Deng, the team is” really precise” at every stage, including heat control, malaria counting, and the strict feeding schedule. He continued,” This is done to achieve the best malaria growth in terms of length and produce value.” & nbsp,
For instance, a basket containing mosquito larvae will fall if there is too much meals. The female mosquito will be smaller if there is not enough meals, making it difficult to tell the difference between the men and the girls. & nbsp,
For on seven times, the mosquito larvae remain in the room. They are harvested on time seven in preparation for gender detachment, the next step. & nbsp,
Separating males from females and nbsp is stage four.
To distinguish between the men and the women after the produce, the larvae are examined based on their size. An AI-based sensor that captures a photo of the sample caterpillars is used for this. & nbsp,
The size of the pupae’s shoulders or the width of their cephalothorax — the females are larger — is used by the scanner to identify them. & nbsp,
1 to 1.5 million female mosquitoes are produced in the sexual processing area over the course of three to four hours.
Shipping and transportation are step five.
The flies are packed and transported for discharge at this point in the production period. & nbsp,
A black pot is used to store about 200 male Wolbachia caterpillars, which are then packed into containers of 35 pots each. The mosquitoes are kept indoors for a few days to fully build because they are still in the pupa stage. & nbsp,
The mosquitoes are then released at different locations after being collected by an NEA area official. & nbsp,